Activation of the JNK signaling pathway: breaking the brake on apoptosis

The JNK signaling pathway is involved in regulation of many cellular events, including growth control, transformation and programmed cell death (apoptosis). The role of JNK activation in apoptosis is highly controversial, being suggested to have a pro-apoptotic, anti-apoptotic or no role in this process. It appears that the JNK pathway functions in a cell-type and stimulus-dependent manner and its different components can sometimes play opposing roles in apoptosis. Recent studies reveal that the effect of JNK activation on apoptosis depends on the activity of other signaling pathways like the NF-kappaB pathway. Here we propose a model that can explain how activation of the JNK pathway "breaks the brake" on apoptosis, thereby regulating, but not initiating the apoptotic process.     

钉螺在枫杨防护林和芦林分布密度的比较研究

通过对长江外滩枫杨防护林和芦林钉螺分布密度的调查比较,结果表明：枫杨林钉螺分布密度明显低于芦林;两种群落钉螺分布密度不同程度受长江洪泛的影响;两种群落中４种抑制钉螺的植物对钉螺分布密度的影响相似。     

4－硒（代）硫酸酯多糖的抗肿瘤免疫调节作用及其机制研究

本实验系统地研究了４－硒（代）硫酸酯多糖的抗肿瘤免疫调节作用及其机制。发现该药在体内外均有抑制肿瘤生长的作用,并认为其抑瘤机制与促进巨噬细胞活性,间接促进淋巴细胞活性,释放具有杀伤肿瘤细胞的效应分子以及选择性抑制肿瘤细胞大分子合成有关。结果表明,４－硒（代）硫酸酯多糖是一种兼具杀伤肿瘤细胞和增强免疫功能双重作用的新型免疫型抗肿瘤药。     

Calcium, polarity and osmoregulation in Fucus embryos: one messenger, multiple messages

This review addresses the role of cytosolic Ca²⁺in the regulation of embryonic polarity, polarised growth and osmotic control of cell volume with particular emphasis on the marine alga Fucus. Evidence is presented that the control of cell elongation in plants and algae requires the co-ordinate control of cell turgor and vesicle exocytosis, all of which are regulated by cytosolic Ca²⁺. In the Fucus embryo, polarised growth of the rhizoid cell requires sustained elevation of Ca²⁺ at the rhizoid apex whereas osmotic cell volume control is effected by transient elevations of cytosolic Ca²⁺. The mechanisms by which these signals are generated and their different downstream responses are discussed. Answers to questions of how specificity of signalling can be achieved by signal-response pathways which share common components will require more detailed knowledge of the interactions between different components and the spatial and temporal patterns of their activation.     

原代小鼠肝脏细胞的高效分离纯化与培养*

摘要目的：建立一种能稳定获得高活力和高纯度原代小鼠肝脏细胞的分离、纯化及培养方法。方法：应用改良的Seglen 二步法原 位灌注和机械离心分离肝脏细胞,并用改良的高糖DMEM培养基进行培养。台盼蓝拒染法检测接种时肝脏细胞的存活率,倒置 显微镜动态观察肝脏细胞形态变化,应用免疫荧光技术对肝脏细胞进行Albumin 染色。结果：每只小鼠可获取肝脏细胞的总产量 平均为1.35× 106 / g体重,肝脏细胞存活率＞ 90%。倒置显微镜下观察贴壁前肝细胞直径为35.14 滋m± 4.35 滋m,肝脏细胞在接种 后3 h基本完成贴壁;肝脏细胞接种后24h,所有肝脏细胞均强阳性表达成熟肝脏细胞标志物Albumin,肝细胞纯度＞ 95%。结 论：改良的分离纯化及培养方法能稳定获得高产量、高活率及高纯度的小鼠肝脏细胞。     

原发性高血压患者尿微量白蛋白和冠状动脉狭窄性病变程度的关系

目的:分析并探讨原发性高血压患者尿微量白蛋白和冠状动脉狭窄性病变程度的关系。方法:选择2010年8月至2013年8月符合条件的患者按照MAU阳性与否分为阳性组和阴性组各98例,分别记录并比较尿微量白蛋白、冠状动脉造影情况及Gensini积分。结果:1阳性组与阴性组患者在性别、年龄、病程、吸烟史、饮酒史、高血脂症史、血肌酐水平比较差异无统计学意义(P0.05)。2两组患者收缩压、舒张压、脉压及脉压指数比较,差异无统计学意义(P0.05)。3阳性组相比阴性组冠状动脉造影阳性率高,双支及三支冠脉病变率亦高,差异有统计学意义(P0.05);且阳性组Gensini积分显著高于阴性组(P0.05)。结论:原发性高血压伴有MAU阳性患者存在相对更严重的冠状动脉狭窄性病变,通过检测MAU指标有助于及时判断患者病情并指导治疗方案。     

唐古特白刺果实化学成分和功效作用研究进展

唐古特白刺是中国特有的白刺属植物,民间用于治疗脾胃虚弱、消化不良、神经衰弱、感冒等症。本文综述了唐古特白刺果实化学成分和功效作用方面的研究进展。     

青海沙棘不同部位总黄酮含量比较研究

研究了青海沙棘不同部位总黄酮含量的差异。用超声提取作为沙棘总黄酮的制备方法,以芦丁为对照品,采用分光光度法作为沙棘总黄酮的测定方法。结果表明:沙棘根、茎和叶中总黄酮含量分别为5.12、11.37和95.87 mg/g,叶中总黄酮含量远高于根和茎。与其他植物和其它地区沙棘叶片中总黄酮的含量相比,青海沙棘叶片中总黄酮含量都较高,因此青海沙棘具有较大的开发价值和应用前景。     

丽江大黄中多羟基芪类成分的提取工艺研究

本文采用正交实验,以Rhaponfin和Desoxyrhaponticin的总含量为指标,运用HPLC含量分析测定法,研究了丽江大黄中多羟基芪类成分的提取工艺.结果表明:以料液比1:12,提取温度65℃,pH =7,浓度为90％乙醇回流提取2h为最佳提取条件,在此条件下提取的多羟基芪类成分Rhapontin和Desoxyrhaponticin的总含量为104.98 mg/g.